Chromatin immunoprecipitation and microarray-based analysis of protein location
نویسندگان
چکیده
منابع مشابه
Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis.
Previously, identification of promoters regulated by mammalian transcription factors has relied upon overexpression studies. Here we present the identification of a large set of promoters that are bound by E2F in physiological conditions. Probing a human CpG microarray with chromatin immunoprecipitated using an antibody to E2F4, we have identified 68 unique target loci; 15% are bidirectional pr...
متن کاملFast chromatin immunoprecipitation assay
Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin...
متن کاملNative chromatin immunoprecipitation.
Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin immunoprecipitation. We describe here a protocol tha...
متن کاملChromatin immunoprecipitation (ChIP).
Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic l...
متن کاملChromatin immunoprecipitation assay.
Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular p...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2006
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2006.98